The transgenic RNAi in flies is the most convenient tool to study the genes in vivo through conditional manner, and as an ideal animal model platform to screen novel modulators in signaling pathways and diseases, as most fly genes are homologous to human, and the majority human disease genes can be found in fly. To improve the knockdown efficacy and to limit position effect and off targets as well, we developed the 2nd and 3rd generation transgenic RNAi techniques. It significantly triggers RNAi not only in soma, but also in germline (Ni et al., 2008, 2011, Nature Methods). TsingHua Fly Center (THFC) is established in 2011, we initiated a large-scale transgenic RNAi lines production at that time, with 500 transgenic lines per month if the hairpin vectors are sequenced and well purified. Currently we are keeping thousands of transgenic lines in house for life science and biomedical research.
Besides expanding the transgenic RNAi resources, we also developed CRISPR/Cas9 genome editing system for the fly community (Ren et al., 2013, PNAS). We reported an effective CRISPR-based method for genome editing in Drosophila. In this system, plasmids encoding single-guide RNAs (sgRNAs) are injected into embryos of germline specific Cas9 transgenic flies. The major features of our system are its high efficiency and ease to apply.
For the transgenic RNAi lines and site-specific genome editing, please send us your request. We also provide certain external compensable service, such as the vector constructions and microinjections.